• Technical Name
  • Virus and Cell Culture Platform for Vaccine Production technology
  • Operator
  • National Pingtung University of Science and Technology
  • Booth
  • Online display only
  • Contact
  • 陳肇閎
  • Email
  • cch1120@mail.npust.edu.tw
Technical Description “Virus and Cell Culture Platform for Vaccine Production Technology” contains:
1.Host cell and virus match and colonization for preparing cell lines suitable for virus production; 2.Cell expresses virus receptor technology for virus quickly infecting cells; 3.Blocking antiviral mechanism efficiently promotes virus replication in host cells through genetic engineering; 4.Immortalization of host cells achieves the purpose of industrialization and stable production capacity; 5. Cell-free serum suspension culture technology removes the possibility of contaminating vaccine. Effectively stabilizing the virus proliferation depends on technical blocking the cell immune response and apoptosis after virus infection. This technique diminishes the immune response and apoptosis under virus infection and proliferation through genetic engineering for silencing specific antiviral gene in host cells. The technique effectively propagates industrial viruses for vaccine development.
Scientific Breakthrough Through gene editing and transfection, these techniques block the host cell's antiviral mechanism, and let the host cells express the specific virial receptor, reduce the immune response and apoptosis after virus infection, and provides rapid propagation of the virus. This technique strengthens the susceptibility of host cells to viruses, increases virus productivity, and produces intact antigens for production of commercial vaccines. These techniques can effectively increase productivity (market technology/high-performance cell production virus system), such as Pseudorabies virus (10^7.5/10^10), Porcine infectious diarrhea virus (10^4.5/10^8.5), Very virulent infectious bursal disease viruses (10^3.5/10^8.5), Newcastle virus (10^7.5/10^9.5), Avian reovirus (10^6.5/10^8.5).
Industrial Applicability The industrial application is as follows: 1. Manufacturing various vaccines. 2. Reducing the cost of vaccine preparation. 3. Virus production with high efficiency: The data shows that it can increase the output by 1000 times than other virus production methods. 4. Industrialization: This technology has been successfully applied to vaccine manufacturing. 5. Low pollution: This process uses culture virus without adding animal serum to diminish possible pollution in the vaccine production. 6. Rapid vaccine production responses to acute animal or human epidemics. 7. Development of antiviral drug screening platform is based on this technique. 8. It adapts animal welfare and humanitarianism and conducive to technology promotion.