A primer set for the detection ofCymbidium mosaic virus and uses thereof are provided. The primer set comprises at least one of primers for amplifying a gene fragment of a coat protein ofCymbidium mosaic virus selected from the group consisting of:
(a) an upstream outer primer, comprising a nucleic acid sequence of SEQ ID NO.1 or SEQ ID NO.2;
(b) an upstream inner primer, comprising a nucleic acid sequence of SEQ ID NO.3;
(c) a downstream outer primer, comprising a nucleic acid sequence of SEQ ID NO.4, SEQ ID NO.5, or SEQ ID NO.6; and
(d) a downstream inner primer, comprising a nucleic acid sequence of SEQ ID NO.7.

A primer set for the detection ofCymbidium mosaic virus and uses thereof are provided. The primer set comprises at least one of primers for amplifying a gene fragment of a coat protein ofCymbidium mosaic virus selected from the group consisting of:
(a) an upstream outer primer, comprising a nucleic acid sequence of SEQ ID NO.1 or SEQ ID NO.2;
(b) an upstream inner primer, comprising a nucleic acid sequence of SEQ ID NO.3;
(c) a downstream outer primer, comprising a nucleic acid sequence of SEQ ID NO.4, SEQ ID NO.5, or SEQ ID NO.6; and
(d) a downstream inner primer, comprising a nucleic acid sequence of SEQ ID NO.7.

本發明係關於一種用於偵測蕙蘭嵌紋病毒(Cymbidium mosaic virus)之引子組合及其應用，尤其係關於使用該引子組合藉由反轉錄-等溫迴圈核酸增幅反應(reverse transcription-loop mediated isothermal amplification，RT-LAMP)以偵測蕙蘭嵌紋病毒之應用。